6E48
Crystal Structure of the Murine Norovirus VP1 P domain in complex with the CD300lf Receptor and Lithocholic Acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2017-10-02 |
Detector | NOIR-1 |
Wavelength(s) | 1.00004 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 101.091, 152.891, 70.037 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 63.674 - 1.801 |
R-factor | 0.1751 |
Rwork | 0.174 |
R-free | 0.20390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB entries 3LQ6 & 5FFL |
RMSD bond length | 0.022 |
RMSD bond angle | 1.109 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 63.680 | 1.870 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 95932 | 11228 |
<I/σ(I)> | 5.92 | 0.65 |
Completeness [%] | 95.0 | 63 |
Redundancy | 2 | 1.8 |
CC(1/2) | 0.996 | 0.324 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.7 | 293 | 0.1 M Tris, pH 8.7, 40 mM calcium chloride, 5.5% PEG10000 |