6E47
Crystal Structure of the Murine Norovirus VP1 P domain in complex with the CD300lf Receptor and Glycochenodeoxycholic Acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2017-10-02 |
Detector | NOIR-1 |
Wavelength(s) | 1.00004 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.105, 134.824, 139.592 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 60.700 - 1.950 |
R-factor | 0.2109 |
Rwork | 0.208 |
R-free | 0.25710 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB entries 3LQ6 & 5FFL |
RMSD bond length | 0.007 |
RMSD bond angle | 0.612 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.700 | 2.020 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.244 | |
Number of reflections | 67388 | 1306 |
<I/σ(I)> | 6.48 | |
Completeness [%] | 87.0 | |
Redundancy | 5.8 | |
CC(1/2) | 0.991 | 0.046 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.7 | 293 | 0.1 M Tris, pH 8.7, 0.11 M magnesium chloride, 11% PEG10000, 100 uM sodium glycochenodeoxycholate |