6E3I

Human Bfl-1 in complex with the Bfl-1-specific designed peptide srt.F4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97910
Spacegroup name	P 1 21 1
Unit cell lengths	43.220, 42.947, 46.762
Unit cell angles	90.00, 114.73, 90.00

Refinement procedure

Resolution	39.255 - 1.481
R-factor	0.142
Rwork	0.139
R-free	0.17290
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.510
High resolution limit [Å]	1.480	4.020	1.480
Rmerge	0.061	0.040	0.344
Rmeas	0.069	0.044	0.411
Rpim	0.030	0.019	0.218
Number of reflections	23986	1282	737
<I/σ(I)>	7.7
Completeness [%]	92.5	94.3	57.4
Redundancy	4.5	4.9	2.7
CC(1/2)		0.998	0.856

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	1.8 M ammonium sulfate, 0.1 M MES pH 7.0