6E39

Capsid protein of PCV2 with 2-O-sulfo-alpha-L-idopyranuronic acid and N,O6-DISULFO-GLUCOSAMINE

This is a large structure.

EM specimen

Vitrificationfalse
Stainingfalse
InstrumentFEI VITROBOT MARK IV

EM Data Collection

MicroscopeFEI TITAN
Electron GunFIELD EMISSION GUN
Accelerating Voltage (kV)300
Cs (mm)0.01
DetectorGATAN K2 SUMMIT (4k x 4k)

3D Reconstruction

MethodSINGLE PARTICLE
Resolution (Å)3.5
Number of Particle166369
Software,Leginon,Gctf,Coot,PHENIX,RELION,FREALIGN
SymmetryI
CTF correctionPHASE FLIPPING AND AMPLITUDE CORRECTION