6E2B
Ubiquitin in complex with Pt(2-phenilpyridine)(PPh3)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-03-25 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.987 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 140.381, 140.381, 62.447 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 99.260 - 1.450 |
R-factor | 0.18234 |
Rwork | 0.181 |
R-free | 0.20735 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ubq |
RMSD bond length | 0.018 |
RMSD bond angle | 2.359 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.260 | 1.480 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.160 | |
Rmeas | 0.168 | |
Rpim | 0.069 | 0.566 |
Number of reflections | 110120 | 5428 |
<I/σ(I)> | 13.8 | 1.58 |
Completeness [%] | 99.0 | 36.7 |
Redundancy | 12.9 | 10.8 |
CC(1/2) | 0.476 | 0.476 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 289 | 3M ammonium sulfate, 0.1M sodium citrate buffer, pH 5.5 |