Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 60.580, 173.410, 215.909 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.280 - 1.900 |
| R-factor | 0.17402 |
| Rwork | 0.172 |
| R-free | 0.21258 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bhl |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.786 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.790 | 0.120 |
| Number of reflections | 40413 | 5400 |
| <I/σ(I)> | 11 | 3 |
| Completeness [%] | 95.3 | 91.3 |
| Redundancy | 5.7 | 5.7 |
| CC(1/2) | 1.000 | 0.820 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 293 | 20% w/v PEG 3350, 0.2 M potassium formate |
