6DZN

Pan-ebolavirus human antibody ADI-15878 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-11
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	42.057, 96.837, 54.309
Unit cell angles	90.00, 102.91, 90.00

Refinement procedure

Resolution	46.449 - 2.100
R-factor	0.2256
Rwork	0.224
R-free	0.26510
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.580
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.450	2.140
High resolution limit [Å]	2.100	2.100
Rmerge	0.170	1.180
Rpim	0.120	0.820
Number of reflections	24142	1150
<I/σ(I)>	6.4	0.9
Completeness [%]	96.9	96.5
Redundancy	2.92	2.82
CC(1/2)	0.730	0.310

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	277.15	0.1 M citric acid pH 5.0, 1 M LiCl and 20% (w/v) polyethylene glycol 6000