6DWO

Crystal structure of alpha-1-2-mannosidase from Enterococcus faecalis V583

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	C 2 2 21
Unit cell lengths	163.350, 168.990, 258.520
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.180 - 2.150
R-factor	0.15698
Rwork	0.155
R-free	0.18893
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5swi
RMSD bond length	0.008
RMSD bond angle	1.511
Data reduction software	XDS (Jun 1, 2017 BUILT=20170601)
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0230)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.210
High resolution limit [Å]	2.150	2.150
Rmerge	0.096	0.678
Rmeas	0.111	0.703
Number of reflections	190871	14034
<I/σ(I)>	11.12	1.93
Completeness [%]	99.0	99.2
Redundancy	4	4
CC(1/2)	0.997	0.782

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	0.15 M sodium acetate, pH 4.5, 0.8 M sodium phosphate monobasic, 1.2 M potassium phosphate dibasic