6DUY
Crystal structure of the alpha-N-catenin actin-binding domain H1 mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-04-15 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 75.094, 82.845, 105.309 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.440 - 2.810 |
| R-factor | 0.2042 |
| Rwork | 0.199 |
| R-free | 0.25480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4k1o |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.926 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.440 | 2.910 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.072 | 0.687 |
| Number of reflections | 16455 | |
| <I/σ(I)> | 21.7 | 2.8 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.3 | 7.4 |
| CC(1/2) | 0.970 | 0.880 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 100 mM sodium acetate/acetic acid, pH 4.5, 0.8 M sodium phosphate monobasic, 1.2 M potassium phosphate dibasic |
