6DUW

Crystal structure of the alpha-N-catenin actin-binding domain H1 mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-15
Detector	RAYONIX MX-300
Wavelength(s)	1
Spacegroup name	P 65 2 2
Unit cell lengths	108.652, 108.652, 133.078
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.080 - 2.200
R-factor	0.204
Rwork	0.202
R-free	0.22630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4k1o
RMSD bond length	0.008
RMSD bond angle	0.789
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.080	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.076	0.680
Number of reflections	24191
<I/σ(I)>	31.7	3.6
Completeness [%]	99.8	100
Redundancy	13.1	13.3
CC(1/2)	0.980	0.890

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	2.0 M ammonium sulfate, 10 mM cobalt chloride