6DUJ
Crystal structure of A51V variant of Human Cytochrome c
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-22 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.07 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 62.380, 184.369, 35.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.323 - 1.822 |
| R-factor | 0.189334445331 |
| Rwork | 0.184 |
| R-free | 0.23488 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ty3 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.248 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.330 | 1.890 |
| High resolution limit [Å] | 1.820 | 1.830 |
| Rmerge | 0.106 | 1.180 |
| Number of reflections | 18770 | 1878 |
| <I/σ(I)> | 24.5 | |
| Completeness [%] | 98.7 | 99 |
| Redundancy | 6.5 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.1 M sodium citrate pH 5.5, 40%(w/v) PEG 600 |
