6DU4

Crystal structure of hMettl16 catalytic domain in complex with MAT2A 3'UTR hairpin 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-09
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97937
Spacegroup name	P 43
Unit cell lengths	74.979, 74.979, 93.213
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.004 - 1.700
R-factor	0.1529
Rwork	0.152
R-free	0.17260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	our own model
RMSD bond length	0.007
RMSD bond angle	0.962
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	MOLREP
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.101	1.421
Number of reflections	56305	5609
<I/σ(I)>	15.9	4.29
Completeness [%]	100.0
Redundancy	12.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M Tris (pH 8.5), 20% glycerol, and 19% polyethylene (PEG) 10000