6DU3
Structure of Scp1 D96N bound to REST-pS861/4 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2015-02-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97648 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 125.414, 78.731, 63.001 |
Unit cell angles | 90.00, 112.42, 90.00 |
Refinement procedure
Resolution | 39.200 - 2.580 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.610 |
High resolution limit [Å] | 2.570 | 6.970 | 2.570 |
Rmerge | 0.082 | 0.031 | 0.384 |
Total number of observations | 66701 | ||
Number of reflections | 17864 | 946 | 690 |
<I/σ(I)> | 7.6 | ||
Completeness [%] | 98.0 | 99.8 | 76.6 |
Redundancy | 3.7 | 3.7 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 30% PEG 3350 0.2 M Magnesium acetate |