6DTN
The structure of NTMT1 in complex with compound DC100-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-21 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 73.166, 73.166, 82.361 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.570 - 1.475 |
| R-factor | 0.1691 |
| Rwork | 0.168 |
| R-free | 0.18930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5e1m |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.187 |
| Data reduction software | HKL-2000 (denzo) |
| Data scaling software | HKL-2000 (scalepack) |
| Phasing software | PHASER (with PHENIX) |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.570 | 1.510 |
| High resolution limit [Å] | 1.470 | 1.470 |
| Rmeas | 0.142 | 4.939 |
| Rpim | 0.062 | 2.158 |
| Number of reflections | 43110 | 3067 |
| <I/σ(I)> | 9.9 | 0.4 |
| Completeness [%] | 99.3 | 97 |
| Redundancy | 9.9 | 9.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.5 M lithium sulfate; 0.1 M sodium HEPES 7.5 |
