6DTK
Heterodimers of FALS mutant SOD enzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.953700 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 162.699, 201.671, 143.834 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.570 - 2.000 |
| R-factor | 0.158 |
| Rwork | 0.156 |
| R-free | 0.19000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fun |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.361 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.570 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.086 | 0.871 |
| Number of reflections | 158995 | |
| <I/σ(I)> | 25.25 | 2.47 |
| Completeness [%] | 99.9 | 98.9 |
| Redundancy | 14.2 | 13.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 2-3 M Ammonium sulfate or Sodium malonate |
