6DTI

Structure of the Thermus thermophilus 30S ribosomal subunit complexed with an unmodifed anticodon stem loop (ASL) of Escherichia coli transfer RNA Arginine 2 (TRNAARG2) bound to an mRNA with an CGU-codon in the A-site and paromomycin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	277
Detector technology	CCD
Collection date	2010-08-17
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97
Spacegroup name	P 41 21 2
Unit cell lengths	404.540, 404.540, 176.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.343 - 3.540
R-factor	0.1969
Rwork	0.197
R-free	0.23570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xnr
RMSD bond length	0.005
RMSD bond angle	0.855
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.8.4_1496)
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.343	3.670
High resolution limit [Å]	3.540	3.540
Rmerge	0.073	0.754
Number of reflections	171713	17375
<I/σ(I)>	12.42	1.51
Completeness [%]	97.1
Redundancy	2.9
CC(1/2)	0.992	0.526

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	MPD, NH4Cl, KCl, CaCl2, magnesium acetate, sodium cacodylate, pH 6.5 VAPOR DIFFUSION, HANGING DROP, temperature 277K, temperature 277.0K