6DPP

Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 5 mM Mg2+ and 200 mM Rb+ for 240 s at 21 C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-24
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	81.468, 37.992, 62.477
Unit cell angles	90.00, 96.81, 90.00

Refinement procedure

Resolution	19.936 - 1.450
R-factor	0.1523
Rwork	0.151
R-free	0.17070
Starting model (for MR)	pdbid 1ZBL
RMSD bond length	0.008
RMSD bond angle	1.019
Data reduction software	HKL-2000
Data scaling software	Aimless (0.5.31)
Phasing software	PHENIX
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.940	19.940	1.470
High resolution limit [Å]	1.450	7.940	1.450
Rmerge	0.026	0.018	0.649
Rmeas	0.037	0.026	0.918
Rpim	0.026	0.018	0.649
Total number of observations	63571	375	2087
Number of reflections	33303	212	1255
<I/σ(I)>	14.3	48.7	1
Completeness [%]	98.1	92.3	77.3
Redundancy	1.9	1.8	1.7
CC(1/2)	0.999	0.998	0.472

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2