6DP5
Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 40 mM Mg2+ and 75 mM K+ for 40 s at 21 C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-22 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 81.899, 37.505, 62.254 |
Unit cell angles | 90.00, 96.48, 90.00 |
Refinement procedure
Resolution | 20.008 - 1.432 |
R-factor | 0.1539 |
Rwork | 0.153 |
R-free | 0.17590 |
Starting model (for MR) | 1zbl |
RMSD bond length | 0.009 |
RMSD bond angle | 1.198 |
Data reduction software | HKL-2000 |
Data scaling software | Aimless (0.5.31) |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.010 | 20.010 | 1.460 |
High resolution limit [Å] | 1.430 | 7.840 | 1.430 |
Total number of observations | 32560 | ||
Number of reflections | 32560 | 218 | 1325 |
<I/σ(I)> | 6.8 | ||
Completeness [%] | 93.8 | 92.3 | 76.4 |
Redundancy | 1 | 1 | 1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 294 | 14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2 |