6DNZ

Trypanosoma brucei PRMT1 enzyme-prozyme heterotetrameric complex with AdoHcy

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-06
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	C 1 2 1
Unit cell lengths	196.582, 65.904, 141.028
Unit cell angles	90.00, 106.90, 90.00

Refinement procedure

Resolution	50.000 - 2.384
R-factor	0.1887
Rwork	0.188
R-free	0.22170
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.522
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	PHENIX ((dev_3071: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.384	2.384
Rmerge	0.103	0.773
Number of reflections	97551	3757
<I/σ(I)>	22.2
Completeness [%]	88.7	54
Redundancy	10.5	3.5
CC(1/2)	0.997	0.651

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	295	7% (w/v) PEG 4000, 0.1 M Tris-HCl, pH 7.4