6DNK
Human Stimulator of Interferon Genes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 110.414, 110.414, 35.863 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.070 - 1.950 |
| R-factor | 0.195 |
| Rwork | 0.193 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6cy7 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.771 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.040 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.073 | 0.718 |
| Number of reflections | 16624 | |
| <I/σ(I)> | 11.1 | 2.1 |
| Completeness [%] | 99.4 | 99.2 |
| Redundancy | 5.4 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.7 | 285 | Sodium sulfate, PEG 3350 |
