6DN8
SPRY domain-containing SOCS box protein 2 complexed with (GZJ)VDINNN(CY3) Cyclic peptide inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-06-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.95370 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 49.020, 109.370, 118.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.270 - 1.750 |
| R-factor | 0.194 |
| Rwork | 0.193 |
| R-free | 0.21000 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.040 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.270 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.081 | 0.822 |
| Rpim | 0.030 | 0.295 |
| Number of reflections | 64838 | |
| <I/σ(I)> | 16.2 | 2.7 |
| Completeness [%] | 99.9 | |
| Redundancy | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 277.15 | 0.2M NH4SO4, 0.1M NaAc, 25% PEG4,000 |
