6DN7
SPRY domain-containing SOCS box protein 2 complexed with WDINNN(BAL) Cyclic peptide inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.95370 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.690, 65.440, 69.180 |
| Unit cell angles | 90.00, 103.60, 90.00 |
Refinement procedure
| Resolution | 32.610 - 1.400 |
| R-factor | 0.198 |
| Rwork | 0.197 |
| R-free | 0.21300 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.020 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.610 | 1.480 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.048 | 0.474 |
| Rpim | 0.031 | 0.324 |
| Number of reflections | 91682 | |
| <I/σ(I)> | 12.7 | 2.4 |
| Completeness [%] | 98.3 | |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.9 | 277.15 | 0.2 M Magnesium chloride hexahydrate 20 % w/v PEG 3350 |
