6DN5
SPRY domain-containing SOCS box protein 2 complexed with WDINNN(BAL) cyclic peptide inhibitor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-04-13 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.95370 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 32.620, 65.950, 110.020 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.240 - 2.400 |
R-factor | 0.178 |
Rwork | 0.174 |
R-free | 0.24600 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.090 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.240 | 2.520 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.225 | 0.687 |
Rpim | 0.090 | 0.286 |
Number of reflections | 9853 | |
<I/σ(I)> | 6.6 | 2.9 |
Completeness [%] | 99.4 | |
Redundancy | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | HEPES pH 7.5, 20% PEG 4000, 10%Isopropanol |