6DKS

Structure of the Rbpj-SHARP-DNA Repressor Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	200
Detector technology	CCD
Collection date	2012-02-08
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.978
Spacegroup name	P 1 21 1
Unit cell lengths	54.450, 231.570, 90.260
Unit cell angles	90.00, 99.88, 90.00

Refinement procedure

Resolution	38.530 - 2.780
R-factor	0.1966
Rwork	0.195
R-free	0.22790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3brg 3h4z
RMSD bond length	0.008
RMSD bond angle	0.960
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.780	2.800
Rmerge	0.084	0.480
Number of reflections	54503	3903
<I/σ(I)>	14.9
Completeness [%]	99.3	95.14
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6.6	277	Bis-Tris pH6.6 100mM NaCl 40% PEG400 200mM NDSB-256