6DGD

PriA helicase bound to dsDNA of a DNA replication fork

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-26
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.1271295
Spacegroup name	P 21 21 21
Unit cell lengths	100.560, 106.760, 256.830
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.343 - 2.823
Rwork	0.247
R-free	0.28990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4NL4 removing the winged helix domain
RMSD bond length	0.013
RMSD bond angle	1.134
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.343	3.080	2.890
High resolution limit [Å]	2.823	3.000	2.810
Rmerge	0.215
Rmeas	0.218	4.283	3.237
Number of reflections	66968	3448	3996
<I/σ(I)>	11.93	0.63	0.7
Completeness [%]	98.6	84.4	80.6
Redundancy	37.9	10.7	10.7
CC(1/2)	0.999	0.266	0.458

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	85 mM sodium citrate-NaOH pH 5.60, 0.25 M ammonium suflate, 0.75 M lithium sulfate, and 4 % glycerol
2	VAPOR DIFFUSION, HANGING DROP	5.6	293	85 mM sodium citrate-NaOH pH 5.60, 0.25 M ammonium suflate, 0.8 M lithium sulfate, 4 % glycerol, 5 mM spermidine tetrahydrochloride, 20 mM praseodymium III acetate hydrate
3	VAPOR DIFFUSION, HANGING DROP	5.6	293	85 mM sodium citrate-NaOH pH 5.60, 0.25 M ammonium suflate, 0.75 M lithium sulfate, 4 % glycerol, 5 mM spermidine tetrahydrochloride, and 0.1-0.2 mM adenosine diphosphate
4	VAPOR DIFFUSION, HANGING DROP	5.6	293	85 mM sodium citrate-NaOH pH 5.60, 0.25 M ammonium suflate, 0.75 M lithium sulfate, 4 % glycerol, 5 mM spermidine tetrahydrochloride, and 0.1-0.2 mM potassium chloride