6DG0
MEC-8 C-terminal RRM domain bound to AGCACA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-X |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-01-01 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 0.97 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 72.075, 68.714, 57.531 |
| Unit cell angles | 90.00, 122.40, 90.00 |
Refinement procedure
| Resolution | 28.082 - 2.457 |
| R-factor | 0.2376 |
| Rwork | 0.235 |
| R-free | 0.28710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1urn |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.779 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.082 | 2.544 |
| High resolution limit [Å] | 2.457 | 2.457 |
| Rmerge | 0.054 | 0.212 |
| Rmeas | 0.076 | |
| Number of reflections | 8221 | 658 |
| <I/σ(I)> | 9.64 | 2.56 |
| Completeness [%] | 94.1 | 76.33 |
| Redundancy | 1.8 | 1.4 |
| CC(1/2) | 0.996 | 0.932 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 1mM MES ph6.5, PEG 8000 25% |
