6DG0

MEC-8 C-terminal RRM domain bound to AGCACA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-X
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-01-01
Detector	DECTRIS PILATUS 200K
Wavelength(s)	0.97
Spacegroup name	C 1 2 1
Unit cell lengths	72.075, 68.714, 57.531
Unit cell angles	90.00, 122.40, 90.00

Refinement procedure

Resolution	28.082 - 2.457
R-factor	0.2376
Rwork	0.235
R-free	0.28710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1urn
RMSD bond length	0.003
RMSD bond angle	0.779
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.082	2.544
High resolution limit [Å]	2.457	2.457
Rmerge	0.054	0.212
Rmeas	0.076
Number of reflections	8221	658
<I/σ(I)>	9.64	2.56
Completeness [%]	94.1	76.33
Redundancy	1.8	1.4
CC(1/2)	0.996	0.932

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	294	1mM MES ph6.5, PEG 8000 25%