6DEZ
Anti-phosphotyrosine antibody PY20-4D5 Fab complexed with sulfate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-01-31 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.11583 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 82.480, 94.930, 170.930 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.999 - 3.200 |
R-factor | 0.2309 |
Rwork | 0.228 |
R-free | 0.29600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bj1 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.243 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA (0.5.21) |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 74.300 | 3.360 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.045 | 1.454 |
Rmeas | 0.373 | 6.731 |
Rpim | 0.143 | 2.758 |
Total number of observations | 397240 | |
Number of reflections | 20773 | 4345 |
<I/σ(I)> | 4.8 | |
Completeness [%] | 99.9 | 99.4 |
Redundancy | 17.9 | 5.7 |
CC(1/2) | 0.993 | 0.105 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 20% PEG4000 and 0.336M ammonium sulfate |