6DDU
mouse beta-mannosidase bound to beta-D-mannose (MANBA)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.03 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.162, 65.651, 104.531 |
| Unit cell angles | 90.00, 92.73, 90.00 |
Refinement procedure
| Resolution | 46.393 - 2.668 |
| R-factor | 0.2026 |
| Rwork | 0.199 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ddt |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.512 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.393 | 2.770 |
| High resolution limit [Å] | 2.668 | 2.670 |
| Number of reflections | 24414 | |
| <I/σ(I)> | 5 | |
| Completeness [%] | 77.2 | |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 9-21% PEG 5000, 0.1 M HEPES |
