6DD5
Crystal Structure of the Cas6 Domain of Marinomonas mediterranea MMB-1 Cas6-RT-Cas1 Fusion Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-11-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9765 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 92.278, 110.760, 192.906 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.226 - 2.850 |
| R-factor | 0.1888 |
| Rwork | 0.187 |
| R-free | 0.22520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5b3w |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.772 |
| Data reduction software | XDS (Jun 17, 2015) |
| Data scaling software | Aimless (0.5.17) |
| Phasing software | PHASER (2.6.0) |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.230 | 48.230 | 2.950 |
| High resolution limit [Å] | 2.850 | 11.040 | 2.850 |
| Rmerge | 0.195 | 0.027 | 1.463 |
| Rmeas | 0.202 | 0.028 | 1.514 |
| Rpim | 0.052 | 0.008 | 0.389 |
| Total number of observations | 696144 | ||
| Number of reflections | 47006 | 915 | 4531 |
| <I/σ(I)> | 17.3 | ||
| Completeness [%] | 100.0 | 99.1 | 100 |
| Redundancy | 14.8 | 13.1 | 15.1 |
| CC(1/2) | 0.997 | 1.000 | 0.766 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.2 | 295 | 0.1 M Sodium Acetate, 2.5 M Ammonium Sulfate |
