6DCW
Crystal structure of human anti-tau antibody CBTAU-27.1 Fab in complex with a human tau peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-01-22 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.979 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 92.308, 59.670, 82.331 |
Unit cell angles | 90.00, 103.62, 90.00 |
Refinement procedure
Resolution | 43.771 - 2.000 |
R-factor | 0.1705 |
Rwork | 0.168 |
R-free | 0.21560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3QOS |
RMSD bond length | 0.005 |
RMSD bond angle | 0.937 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rpim | 0.060 | 0.250 |
Number of reflections | 27347 | |
<I/σ(I)> | 19.5 | 3.5 |
Completeness [%] | 91.5 | |
Redundancy | 4 | |
CC(1/2) | 0.990 | 0.784 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1 M Hepes, pH 7.0, 0.1 M KCl, 15% PEG5000 MME. |