Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-09 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 109.419, 109.419, 97.127 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.934 - 2.444 |
| R-factor | 0.1923 |
| Rwork | 0.189 |
| R-free | 0.22860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2p1l |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.796 |
| Data reduction software | XDS (vNov 11, 2017) |
| Data scaling software | XDS (vNov 11, 2017) |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.934 | 2.505 |
| High resolution limit [Å] | 2.444 | 2.444 |
| Number of reflections | 22390 | |
| <I/σ(I)> | 11.13 | |
| Completeness [%] | 99.7 | |
| Redundancy | 10.8 | |
| CC(1/2) | 0.998 | 0.533 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 2M NaCl, 10% glycerol |
