6DC5

RSV prefusion F in complex with AM22 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X6A
Synchrotron site	NSLS
Beamline	X6A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-31
Detector	ADSC QUANTUM 270
Wavelength(s)	1.000
Spacegroup name	P 21 2 21
Unit cell lengths	132.450, 152.180, 202.930
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.231 - 3.500
Rwork	0.217
R-free	0.28010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mms 6dc4
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.550	3.610
High resolution limit [Å]	3.500	3.500
Rmerge		0.903
Number of reflections	52333	4425
<I/σ(I)>	5.7	2
Completeness [%]	99.8	98.6
Redundancy	5.6
CC(1/2)	0.933

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	32% PEG 400, 4% PEG 3350, 0.01 M cadmium chloride, 0.1 M sodium acetate pH 5.5