6DC3

RSV prefusion F bound to RSD5 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-03-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	I 2 3
Unit cell lengths	270.320, 270.320, 270.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.229 - 3.501
R-factor	0.1838
Rwork	0.183
R-free	0.20500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mmu 1dfb 1mco
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.230	38.230	3.640
High resolution limit [Å]	3.500	12.620	3.500
Rmerge	0.104	0.035	0.846
Rmeas	0.117	0.040	0.960
Rpim	0.053	0.019	0.448
Total number of observations	198500	3891	21266
Number of reflections	41327	906	4656
<I/σ(I)>	9.7	23.5	1.8
Completeness [%]	99.9	95.5	100
Redundancy	4.8	4.3	4.6
CC(1/2)	0.995	0.997	0.528

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.8 M Lithium sulfate, 0.1 M Tris pH 8.0