6DBY

Crystal structure of Nudix 1 from Arabidopsis thaliana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-01-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	36.190, 73.070, 116.540
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	61.908 - 2.000
R-factor	0.1878
Rwork	0.186
R-free	0.22140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4kyx
RMSD bond length	0.007
RMSD bond angle	0.800
Data reduction software	MOSFLM (7.1.0)
Data scaling software	Aimless (0.3.11)
Phasing software	MOLREP
Refinement software	PHENIX ((1.12_2829))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.908	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.106	0.206
Number of reflections	21377	1404
<I/σ(I)>	12.7
Completeness [%]	98.6	90.2
Redundancy	6.4	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	277	0.1 M succinic acid pH 5.0 20% PEG 4000 0.3 M magnesium nitrate