6DBP
RNA-recognition motif 1 of human MSI2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-14 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97920 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 30.317, 64.413, 38.387 |
| Unit cell angles | 90.00, 108.87, 90.00 |
Refinement procedure
| Resolution | 28.687 - 1.603 |
| R-factor | 0.1779 |
| Rwork | 0.174 |
| R-free | 0.21060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uaw |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.863 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rpim | 0.028 | 0.163 |
| Number of reflections | 16779 | 1112 |
| <I/σ(I)> | 21.4 | 3 |
| Completeness [%] | 91.5 | 60.6 |
| Redundancy | 2.7 | 1.8 |
| CC(1/2) | 0.997 | 0.908 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1mM DTT, pH 7.5 |
