6DB7

Crystal structure of anti-HIV-1 V3 Fab 1334 in complex with a HIV-1 gp120 V3 peptide from MN strain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-29
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92009
Spacegroup name	P 1 21 1
Unit cell lengths	70.802, 69.630, 97.191
Unit cell angles	90.00, 97.47, 90.00

Refinement procedure

Resolution	29.168 - 2.213
R-factor	0.2154
Rwork	0.213
R-free	0.26020
RMSD bond length	0.002
RMSD bond angle	0.545
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.170	2.293
High resolution limit [Å]	2.213	2.213
Rmerge	0.100	0.844
Number of reflections	46644
<I/σ(I)>	8.7
Completeness [%]	99.3
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292	0.1 M Na Citrate pH 4.5, 2.0 M (NH4)2SO4, 5% PEG400