6DAQ

PhdJ bound to substrate intermediate

Experimental procedure

Experimental method	LAUE
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2015-08-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.99-1.5
Spacegroup name	P 1 21 1
Unit cell lengths	52.532, 137.200, 88.270
Unit cell angles	90.00, 98.91, 90.00

Refinement procedure

Resolution	45.290 - 2.000
R-factor	0.161
Rwork	0.160
R-free	0.19400
RMSD bond length	0.004
RMSD bond angle	0.667
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Number of reflections	77204
<I/σ(I)>	14
Completeness [%]	93.5
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298.15	0.1 M MES at pH 7-7.5 and 22-25% in PEG 3350