6DAE

2.0 Angstrom crystal structure of the D95V Ca/CaM:CaV1.2 IQ domain complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.033
Spacegroup name	P 21 21 21
Unit cell lengths	65.148, 69.615, 86.078
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.039 - 2.000
R-factor	0.1788
Rwork	0.176
R-free	0.22610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 2BE6 & 4CDK
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.039	43.040	2.120
High resolution limit [Å]	2.000	5.960	2.000
Rmeas	0.134		0.645
Number of reflections	51221		8302
<I/σ(I)>	9.84		2.6
Completeness [%]	99.9		99.7
Redundancy	3.88
CC(1/2)	0.995		0.790

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	10.3	277	1 M lithium chloride, 30% PEG6000, 0.1M Bicine, pH 10.3