6DAD

1.65 Angstrom crystal structure of the N97I Ca/CaM:CaV1.2 IQ domain complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	65.273, 69.045, 85.792
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.511 - 1.650
R-factor	0.1818
Rwork	0.180
R-free	0.20840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6dae
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	41.511	41.511	1.750
High resolution limit [Å]	1.650	4.930	1.650
Rmeas	0.057		0.601
Number of reflections	90091		14567
<I/σ(I)>	14.82		2.17
Completeness [%]	99.9		99.7
Redundancy	3.92
CC(1/2)	0.999		0.804

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	2.1 M sodium malonate, 0.1 M HEPES, pH 7.5