6D9S
The (p)ppGpp-bound crystal structure of HPRT (hypoxanthine phosphoribosyltransferase)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-10 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 82.611, 82.611, 174.916 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.200 - 2.105 |
| R-factor | 0.2037 |
| Rwork | 0.202 |
| R-free | 0.24390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3h83 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.812 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmeas | 0.164 | |
| Rpim | 0.060 | 0.489 |
| Number of reflections | 40594 | 3928 |
| <I/σ(I)> | 16.41 | 1.22 |
| Completeness [%] | 99.6 | 99 |
| Redundancy | 8.1 | 6.2 |
| CC(1/2) | 0.724 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 293.15 | 0.2 M ammonium tartrate dibasic, pH 6.6, 20% PEG3350 |
