6D9S
The (p)ppGpp-bound crystal structure of HPRT (hypoxanthine phosphoribosyltransferase)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-04-10 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97857 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 82.611, 82.611, 174.916 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.200 - 2.105 |
R-factor | 0.2037 |
Rwork | 0.202 |
R-free | 0.24390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3h83 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.812 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmeas | 0.164 | |
Rpim | 0.060 | 0.489 |
Number of reflections | 40594 | 3928 |
<I/σ(I)> | 16.41 | 1.22 |
Completeness [%] | 99.6 | 99 |
Redundancy | 8.1 | 6.2 |
CC(1/2) | 0.724 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 293.15 | 0.2 M ammonium tartrate dibasic, pH 6.6, 20% PEG3350 |