6D9M
T4-Lysozyme fusion to Geobacter GGDEF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-09-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.115820, 2.254180 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 70.677, 111.645, 55.336 |
| Unit cell angles | 90.00, 122.75, 90.00 |
Refinement procedure
| Resolution | 35.747 - 1.350 |
| R-factor | 0.1548 |
| Rwork | 0.154 |
| R-free | 0.17010 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.564 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.27) |
| Phasing software | SOLVE |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.750 | 1.370 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.043 | 0.374 |
| Rmeas | 0.046 | 0.439 |
| Rpim | 0.017 | 0.222 |
| Total number of observations | 512201 | 8093 |
| Number of reflections | 77765 | |
| <I/σ(I)> | 21.6 | 2.9 |
| Completeness [%] | 97.8 | 67.7 |
| Redundancy | 6.6 | 3.1 |
| CC(1/2) | 0.999 | 0.862 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 26% PEG 4,000; 0.03M HEPES-KOH, pH 7.5; 0.3M Na(OAc) |
