6D7G
Structure of 5F3 TCR in complex with HLA-A2/MART-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-07-08 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.91940 |
Spacegroup name | P 62 |
Unit cell lengths | 123.953, 123.953, 154.156 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.300 - 2.750 |
R-factor | 0.212 |
Rwork | 0.211 |
R-free | 0.23700 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.313 |
Data reduction software | DENZO |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.849 |
High resolution limit [Å] | 2.750 | 2.750 |
Rmerge | 0.097 | 0.975 |
Rmeas | 0.102 | 1.025 |
Rpim | 0.032 | 0.315 |
Number of reflections | 34834 | |
<I/σ(I)> | 11.66 | |
Completeness [%] | 99.9 | |
Redundancy | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295.15 | PEG 550 MME, TRIS, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 295K |