6D5V
Ras:SOS:Ras in complex with a small molecule activator
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-03-21 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.98 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 182.822, 182.822, 177.866 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.854 - 2.040 |
R-factor | 0.1673 |
Rwork | 0.167 |
R-free | 0.18620 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4nyi |
RMSD bond length | 0.008 |
RMSD bond angle | 1.062 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.080 |
High resolution limit [Å] | 2.040 | 2.050 |
Rpim | 0.029 | 0.204 |
Number of reflections | 95040 | 4687 |
<I/σ(I)> | 22.6 | |
Completeness [%] | 100.0 | |
Redundancy | 18.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M sodium acetate, 2.0 M sodium formate, pH 4.0 |