6D30

Structure of human Usb1 with uridine-uridine, inactive H208Q mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2017-04-25
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9787
Spacegroup name	P 1 21 1
Unit cell lengths	41.698, 53.038, 45.814
Unit cell angles	90.00, 105.72, 90.00

Refinement procedure

Resolution	44.100 - 1.170
R-factor	0.1503
Rwork	0.149
R-free	0.17170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5v1m
RMSD bond length	0.014
RMSD bond angle	1.297
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.040	1.190
High resolution limit [Å]	1.170	1.170
Number of reflections	60998
<I/σ(I)>	13.7
Completeness [%]	94.1
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	100 mM Tris, 25% SOKALN CP 42, 10% tetrahydrofuran