6D1V

Crystal structure of E. coli RppH-DapF complex, monomer bound to RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-19
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97910
Spacegroup name	C 2 2 21
Unit cell lengths	160.644, 191.986, 51.207
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	61.601 - 1.810
R-factor	0.1994
Rwork	0.199
R-free	0.21960
RMSD bond length	0.008
RMSD bond angle	1.036
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	123.200	1.850
High resolution limit [Å]	1.810	1.810
Rmerge	0.037	0.989
Number of reflections	70344
<I/σ(I)>	12.6
Completeness [%]	96.8
Redundancy	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		292	30% (v/v) PEG400 and 0.1 M CHES, pH 9.2