6D12
Crystal structure of C-terminal xRRM domain of human Larp7 bound to 7SK stem-loop 4 RNA
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979300 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.910, 34.030, 95.010 |
Unit cell angles | 90.00, 95.18, 90.00 |
Refinement procedure
Resolution | 94.622 - 2.205 |
R-factor | 0.2299 |
Rwork | 0.228 |
R-free | 0.26730 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 0.999 |
Data reduction software | XDS (20170615) |
Data scaling software | XSCALE (20170615) |
Phasing software | HKL2Map (2016/1) |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 94.622 | 94.622 | 2.260 |
High resolution limit [Å] | 2.200 | 9.830 | 2.200 |
Rmerge | 0.169 | 0.091 | 1.191 |
Rmeas | 0.181 | 0.098 | 1.279 |
Total number of observations | 120222 | ||
Number of reflections | 15929 | 207 | 1187 |
<I/σ(I)> | 6.54 | 17.27 | 1.43 |
Completeness [%] | 99.9 | 99.5 | 99.9 |
Redundancy | 7.547 | 6.604 | 7.48 |
CC(1/2) | 0.996 | 0.998 | 0.720 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 293 | 0.05M Na Cacodylate,0.08M Mg acetate, 24% PEG 4000 |