6D0X
Crystal structure of chimeric H.2140 / K.1210 Fab in complex with circumsporozoite protein NANP3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-02-24 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.03327 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 83.113, 83.113, 157.166 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.291 - 1.849 |
R-factor | 0.1803 |
Rwork | 0.179 |
R-free | 0.20930 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.211 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.950 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.068 | 0.755 |
Rpim | 0.020 | 0.223 |
Number of reflections | 47923 | 6923 |
<I/σ(I)> | 21.8 | 1.7 |
Completeness [%] | 99.7 | 98.5 |
Redundancy | 12.6 | 12.2 |
CC(1/2) | 0.999 | 0.766 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 294 | 0.1M MES pH 6.5, 20% (w/v) PEG4000, 0.6M NaCl |