6D01
Crystal structure of 1210 Fab in complex with circumsporozoite protein NANP5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-01-14 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.97949 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 205.963, 150.856, 134.651 |
| Unit cell angles | 90.00, 94.81, 90.00 |
Refinement procedure
| Resolution | 39.866 - 3.200 |
| R-factor | 0.2046 |
| Rwork | 0.204 |
| R-free | 0.22670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.526 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.300 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.147 | 0.633 |
| Rpim | 0.088 | 0.375 |
| Number of reflections | 67565 | 5890 |
| <I/σ(I)> | 8.9 | 1.6 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 3.8 | 3.8 |
| CC(1/2) | 0.990 | 0.570 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 20% (w/v) PEG3350, 0.2M sodium citrate |
