6CZZ
Crystal structure of Arabidopsis thaliana phosphoserine aminotransferase isoform 1 (AtPSAT1) in complex with PLP-phosphoserine geminal diamine intermediate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-09 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 189.668, 53.288, 137.921 |
Unit cell angles | 90.00, 91.91, 90.00 |
Refinement procedure
Resolution | 44.400 - 1.700 |
R-factor | 0.1812 |
Rwork | 0.181 |
R-free | 0.20870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4xk1 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.305 |
Data reduction software | XDS (May 1, 2016) |
Data scaling software | XDS (May 1, 2016) |
Phasing software | PHASER (2.5.6) |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.400 | 1.800 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.080 | 0.554 |
Number of reflections | 149284 | 23323 |
<I/σ(I)> | 10.09 | 1.83 |
Completeness [%] | 98.1 | 95.6 |
Redundancy | 3.8 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 292 | 0.2 M lithium sulfate, 17% PEG 3350 and 0.1 M Tris at pH 8.5 |