6CYH
Hsp90-alpha N-domain bound to NEACA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 64.426, 88.873, 99.001 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.580 - 1.490 |
| R-factor | 0.164393434107 |
| Rwork | 0.164 |
| R-free | 0.18179 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yer |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.041 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (1.9_1692) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.530 |
| High resolution limit [Å] | 1.490 | 1.490 |
| Rmerge | 0.050 | 0.413 |
| Number of reflections | 92983 | |
| <I/σ(I)> | 32.6 | 2.1 |
| Completeness [%] | 99.8 | 97.4 |
| Redundancy | 6.1 | 3.5 |
| CC(1/2) | 0.818 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 277 | Bis-Tris Propane pH 6.4, MgCl2, PEG 2000 MME. Soak crystals with NEACA |
